Anisatin is an extremely toxic, insecticidally active component of the Shikimi plant. It is used in folk remedies topically in Japan. Lethal dose = 1 mg/kg (i.p.) in mice. Symptoms begin to appear about 1–6 hours after ingestion, beginning with gastrointestinal ailments, such as diarrhea, vomiting, and stomach pain, followed by nervous system excitation, seizures, loss of consciousness, and respiratory paralysis, which is the ultimate cause of death.