回补反应（英语：Anaplerotic reactions，也称补给反应或添补反应）是指形成代谢途径中间产物的反应。这样的例子可以是三羧酸循环。在该循环为呼吸作用而行使正常作用时，三羧酸循环的中间产物量会保持恒定；然而许多生物合成反应也会使用这些分子作为底物。补给的作用就是将那些为生物合成而被抽出的中间产物补充回来。

三羧酸循环是新陈代谢的中心，在能量产生和生物合成中都起着关键作用。因此，调节线粒体中三羧酸循环的代谢产物对细胞来说就显得非常重要。补给流必须与抽出流相平衡，以保持细胞代谢的稳态。

Anaplerotic reactions (from the Greek ἀνά= 'up' and πληρόω= 'to fill') are chemical reactions that form intermediates of a metabolic pathway. Examples of such are found in the citric acid cycle (TCA cycle). In normal function of this cycle for respiration, concentrations of TCA intermediates remain constant; however, many biosynthetic reactions also use these molecules as a substrate. Anaplerosis is the act of replenishing TCA cycle intermediates that have been extracted for biosynthesis (in what are called cataplerotic reactions).