Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.
释放出的乳酸脱氢酶存在于培养物上清中,可用酶联检测法30分钟测量。这种酶联分析导致四唑盐（INT）转化成红色甲臜化合物。颜色形成的量与裂解细胞的数目成正比。