尼古拉斯维尔（Nicholasville）是美国的一座城市。是肯塔基州杰萨曼县的县治，也是肯塔基州第11大的城市。据2010年人口普查，尼古拉斯维尔有人口28,015人。许多在列克星敦工作的通勤者住在尼古拉斯维尔，也是肯塔基州中部的购物中心及就业中心。

Nicholasville is a home rule-class city in Jessamine County, Kentucky, in the United States. It is the seat of its county. The population was 28,015 during the 2010 U.S. Census, making Nicholasville the 11th-largest settlement in the state. In the last few decades, Nicholasville has experienced rapid growth; the population increased 380% between 1970 and 2010. An official city report published in 2009 declared that "the Welcome mat is out in Nicholasville" for new residents and businesses, and claimed this growth to be "the envy of other cities across the state." The city serves as both a residential area for Lexington-area commuters and as an employment and shopping center for central Kentucky.