Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.

以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。

During two cooling methods, whether tension stress or compression stress, the retained austenite in steel reduced with stress increasing, had become from massive to streak or filminess.

在两种冷却方式下，不论拉应力还是压应力的作用，均使组织中残余奥氏体数量减少，形貌由无应力时的块状变为条状或薄膜状。