Cholesterol oxidase

In enzymology, a cholesterol oxidase (EC1.1.3.6) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are cholesterol and O₂, whereas its two products are cholest-4-en-3-one and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.