非洲兽总目（Afrotheria）是哺乳动物下的一个分支，包含了金毛鼹科、象鼩科、马岛猬科、土豚、蹄兔目、象及海牛。

非洲兽总目的成立主要是基于分子及DNA分析。于1990年代前的基因分析将非洲兽总目看为真兽下纲下的四个大类之一，而牠们之间的关系却倍受争议。其中一个学说指非洲兽总目与其他三个分支是最古老的分裂，发生于一亿零五百万年前非洲从主要大陆分裂时。基因分析及化石纪录显示贫齿总目是在南美洲演化，并分化成劳亚兽总目及灵长总目。劳亚兽总目及灵长总目之间较为接近，并组成北方兽类（Boreoeutheria）。

Afrotheria is a clade of mammals, the living members of which belong to groups that are either currently living in Africa or of African origin: golden moles, elephant shrews (also known as sengis), tenrecs, aardvarks, hyraxes, elephants, sea cows, and several extinct clades. They share few anatomical features but many are partly or entirely African in their distribution. This probably reflects the fact that Africa was an island continent through the early Cenozoic. Because the continent was isolated by water, Laurasian groups such as insectivores, rabbits, carnivorans and ungulates could not become established. Instead, the niches occupied by those groups were filled by tenrecs, hyraxes and elephants that evolved from the ancestral afrothere. This adaptive radiation may have occurred in response to the Cretaceous–Paleogene mass extinction.