Amplified fragment length polymorphism

（重定向自AFLPs）

Example of AFLP data from a capillary electrophoresis instrument

AFLP phylogeny analysis using a dendrogram

AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing polyacrylamide gels, either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.

词汇	AFLPs
分类	英语词汇英语翻译词典
释义	AFLPs 英语百科 Amplified fragment length polymorphism （重定向自AFLPs） AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing polyacrylamide gels, either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.
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