阿亚库乔港是委内瑞拉的城镇，也是亚马逊州的首府，位于该国中部奥里诺科河畔，始建于1924年，面积727平方公里，海拔高度74米，2008年人口约41,000。

Puerto Ayacucho (Spanish pronunciation: [ˈpweɾto aʝaˈkutʃo]) is the capital and largest city of Amazonas State in Venezuela. Puerto Ayacucho is located across the Orinoco River from the Colombian village of Casuarito.

The city was founded to facilitate the transport of goods past the Atures Rapids on the Orinoco River in the late 19th century (mostly rubber). Now the economy is supported by both national and international tourism. Also based here is the Venezuelan army and navy, conducting a continuous low level campaign against incursions and drug-runners from nearby Colombia. The climate is equatorial and the surrounding rainforests are some of the worlds least explored and most untouched. The nearby forested mountains (Tepuis) contain some of the worlds least investigated micro systems.