四眼鱼科为辐鳍鱼纲鲤齿目的其中一科。

四眼鱼科下分3个属，如下：

Anablepidae is a family of freshwater and brackish water fishes living in brackish and freshwater habitats from southern Mexico to southern South America. There are three genera with sixteen species: the four-eyed fishes (genus Anableps), the onesided livebearers (genus Jenynsia) and the white-eye, Oxyzygonectes dovii. Fish of this family eat mostly insects and other invertebrates.