Hook effect
（重定向自Prozone phenomenon）
![Illustration of hook effect adapted from Schiettecatte et al.[1]](/Images/godic/202502/05/Hook_effect0846.png")
![Illustration of the effects of excess antigen and blocking antibodies on immunoassays.](/Images/godic/202502/05/Illustration_of_the_effects_of_excess_antigen_and_blocking_antibodies_on_immunoassays0846.png")
The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or inaccurately low results. Other common forms of interference include antibody interference, cross-reactivity and signal interference. The phenomenon is caused by very high concentrations of a particular analyte or antibody and is most prevalent in one-step (sandwich) immunoassays.