Two-dimensional gel electrophoresis
![2D-Gels (Coomassie stained)](/Images/godic/202412/13/Coomassie-2D-Gels3110.jpg")
![Robots are used for the isolation of protein spots from 2D gels in modern laboratories.](/Images/godic/202412/13/Spot_cutting_and_pipetting_robot_multilanguage3110.jpg")
![Warping: Images of two 2D electrophoresis gels, overlaid with Delta2D. First image is colored in orange, second one colored in blue. Due to running differences, corresponding spots do not overlap.](/Images/godic/202412/13/2D_gel_images_dual_channel_original3110.PNG")
![Warping: Images of two 2D electrophoresis gels after warping. First image is colored in orange, second one colored in blue. Corresponding spots overlap after warping. Common spots are colored black, orange spots are only present (or much stronger) on the first image, blue spots are only present (or much stronger) on the second image.](/Images/godic/202412/13/2D_gel_images_dual_channel_warped3110.PNG")
Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was first independently introduced by O'Farrell and Klose in 1975.