Murlo is a comune (municipality) in the Province of Siena in the Italian region Tuscany, located about 70 kilometres (43mi) south of Florence and about 20 kilometres (12mi) south of Siena.
Murlo borders the following municipalities: Buonconvento, Civitella Paganico, Montalcino, Monteroni d'Arbia, Monticiano, Sovicille.