Immunoscreening

Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene.

Clones are screened for the presence of the gene product (a protein) This strategy requires that a. the library is made in an expression vector b. antiserum to the protein is available Radioactivity or an enzyme is coupled generally with the secondary antibody The radioactivity/enzyme linked secondary antibody -can be purchased commercially -can detect different antigens In commercial diagnostics labs, labelled primary antibodies are also used.