HindIII
![](/Images/godic/202501/18/2e523012.png")
![HindIII restrictions process results in formation of overhanging palindromic sticky ends.](/Images/godic/202501/18/HindIII_Restriction_site_and_sticky_ends_vector.svg3012.png")
![BglII catalytic site, showing the coordination of Asp 84 and Mg2+ with water[3]](/Images/godic/202501/18/BglII_catalytic_site_23012.png")
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg via hydrolysis.
The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends: