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词汇 Genomic library
分类 英语词汇 英语翻译词典
释义

Genomic library

原声例句
2017级专外有声

Genomic libraries are prepared from random fragments of genomic DNA.

然而,基因组文库作为寻找一种特定基因的方法效率并不高,尤其对于含有大量非编码DNA的真核基因组中。

2017级专外有声

However, genomic libraries may be an inefficient method of finding a gene, particularly in large eukaryotic genomes, where much of the DNA is noncoding.

替代的方法是利用已知表达目的基因的细胞或组织来源的mRNA所构建的文库。

中文百科

CDNA文库

cDNA文库筛选在基因功能研究中的应用较早,然而早期的cDNA文库均为「混合型」(pooled),筛选后的回收鉴定工作非常繁杂。近年来由于人类及其它一些物种的全基因组测序工作的完成,使构建基因组范围「cDNA阵列文库」的可能性大为提高。

英语百科

Genomic library CDNA文库

Genomic Library Construction
Colony Blot Hybridization
bac vector
Whole genome shotgun sequencing versus Hierarchical shotgun sequencing

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.

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