柱色谱法（英语：Column chromatography，又称为柱层析），在化学中是最为常用的用来从混合物中分离出纯净物的分离方法，它可以分离尺度跨越毫克到公斤。柱色谱的主要优点是相对的低成本和可处理的固定相，后者可以避免重复利用的交叉污染和固定相的分解。

Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to recycling.