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词汇 DCPIP
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释义

DCPIP

中文百科

二氯酚靛酚 Dichlorophenolindophenol

(重定向自DCPIP)
Z-Schema der Photosynthese mit möglichen Angriffspunkten

2,6-二氯酚靛酚英语:2,6-dichlorophenol Indophenol,简称DCPIPDPIP)为用于氧化还原指示剂的化合物。当DCPIP处于氧化态时呈现蓝色,最大吸光值为600奈米;还原态时则为无色。

DCPIP可用于侦测光合作用的反应速率,为希尔反应家族之一。当该指示剂暴露于光合作用系统时,会因化学还原反应而脱色。DCPIP比起铁氧还蛋白拥有较高电子亲合能,可被光合作用的电子传递链还原,作为光合作用中最终电子载体NADP的替代品。当DCPIP因还原反应而逐渐脱色时,可经由分光光度法测量到其透光率上升。

英语百科

Dichlorophenolindophenol 二氯酚靛酚

(重定向自DCPIP)
Z-Schema der Photosynthese mit möglichen Angriffspunkten

2,6-Dichlorophenolindophenol (DCPIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.

DCPIP can be used to measure the rate of photosynthesis. It is part of the Hill reagents family. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction. DCPIP has a higher affinity for electrons than ferredoxin and the photosynthetic electron transport chain can reduce DCPIP as a substitute for NADP, that is normally the final electron carrier in photosynthesis. As DCPIP is reduced and becomes colorless, the resultant increase in light transmittance can be measured using a spectrophotometer.

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